Whole Genome Sequencing
Fundamental improvement was made for genome sequencing since the next-generation sequencing (NGS) came out in the 2000s. The newer technologies make use of the power of massively-parallel short-read DNA sequencing, genome alignment and assembly methods to digitally and rapidly search the genomes on a revolutionary scale, which enable large-scale whole genome sequencing (WGS) accessible and practical for researchers. Nowadays, whole genome sequencing is more and more prevalent in detecting the genetics of diseases, studying causative relations with cancers, making genome-level comparative analysis, reconstruction of human population history, and giving clinical implications and instructions.
The knowledge of DNA sequences, of the genome of organisms has become inevitable for basic research. Next generation sequencing has overcome barriers and limitations of conventional Sanger sequencing. NGS has made a major impact in genomic sciences and pharmacogenomic research. High throughput technologies and technological advances have significantly reduced the cost and time for a complete analysis of the whole genome. The increased availability of high quality genome sequences for various species offer new opportunities for studying genetic and structural variations like, Single Nucleotide Polymorphism (SNPs), Copy Number Variations (CNVs), insertions, deletions, gene ontology, pathway analysis and other genome wide association studies (GWAS) that can affect gene regulatory pathways and signaling networks.
Unipath Genomics provides Whole Genome Sequencing services on various platforms like Illumina Novaseq, Oxford Nanopore and Sequel Pacbio including project scope, technology selection, data requirements, data generation to high quality draft genome assembly and submission support. We use hybrid approach to achieve maximum genome coverage using mixture of long and short read sequencing, which enhances chances of better coverage for bigger genome projects..
- Generation of quality data for
- Animals
- Plants
- Bacteria
- Virus
- Fungus
- Mitochondrial Genomes
- Unigenomic offers both denovo and reference based sequencing using multiple set of libraries like Paired End and Mate Paired libraries using various NGS technologies.
- For non-model organism denovo genome assembly is performed on High Performing Computing Cluster (HPCC) using high end bioinformatics pipeline to assemble genome into contigs and scaffolds
- The in-house pipeline is used to reduce N's in assembled scaffolds by using the paired end reads. Comprehensive annotation pipeline integrated with various functional and structural databases like NCBI nr, uniprotKB, JCVI_CMR, TIGR and COG databases is used to decipher the genome
- For model organism reference based sequencing is perform on HPCC using specialized tools and software like BWA / Bowtie / gsMapper /SOAPaligner and CLC Genomics Workbench to map reads on reference genome and SNP discovery.
